Regulatory

Part:BBa_K252000:Design

Designed by: Graham Heimberg   Group: iGEM09_Illinois   (2009-10-21)


MicA small RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Attached to the lac promoter sequence (BBa_R0010) with a blunt end restriction site. Restriction enzyme used for blunt end cutting is BfrBI (ATG/CAT).


Source

E. coli MG-1655

References